I want to transfer a RCF that I found in a protocol to RPM. But my centrifuge has offered me two kinds of RCF, the average and maximum. I am confused which one should be used. So hope someone can help me. Thanks.
Given that most centrifuges only give you the maximum RCF (in xG), and vary in acceleration rates, then I have always assumed that is the normal. You might want to specify max G to clarify, but I shouldn't think that necessary.
Different rotors will have different values for average and maximum RCF depending on the length and angle of the sample tube. The average will be the value at the middle of a full tube, and since in many applications tubes do not need to completely filled, the average becomes a bit deceptive. I'd argue that maximum should be reported, and for applications where centrifugation is a critical step, it is on the authors to make the distinction.
Thanks a lot, Justin. Your response is helpful. What i want to let you know is that i am going to do my experiment using ultracentrifuge.As you know, in the situation of ultracentrifugation, tubes are required to be completely filled or nearly filled. The volume of my tubes is about 10ml, so different kinds of RCF matter. Someone told me that most people write their protocols using the average RCF, but i am not that sure.
Thanks,Grant. I am confused which kind of rotor diameter should i take to work it out, the maximum or average. As you know, I am going to do ultracentrifugation. So it matters.