For adipose tissue in Rats, how can I do fixation of the tissue before embedding for making frozen blocks?

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Kai Sun

We just incubate the fat pads in 10% Buffered formalin phosphate for about 24 hrs to 48 hrs (based the size of the adipose tissue).

Patrick Olson

How much buffered formalin phosphate do you use compared to the amount of tissue?

You may soak the fat chunk in the formalin

The bigger the fat chunk the more formalin you should use. I am just having problems with fixing my tissue and I think I didn't use enough volume of formalin and I was wondering if you use a certain volume / tissue.

Thanks for your feedback.

For frozen sections, I used ethanol (90%) for 10 minutes at room temperature.