What is commonly done to render the enzyme inactive, ie, a mutation in the ATP binding domain, etc...
If you just want to inhibit the activity of the enzyme, the easiest way would be to add EDTA in excess of the Mg2+ concentration in the reaction.
If you just want to inhibit the activity of the enzyme, the easiest way would be to add EDTA in excess of the Mg2+ concentration in the reaction.
I do not know if these suggestions are common approaches. (1) You could use the helicase inhibitor Amenamevir (ASP2151). (2) If you think carefully about helicase assay, then you could actually inhibit helicase activity with 20 μl of a solution containing 10 mM Tris-HCl [pH8], 5 mM EDTA, 5 μM cold competitor [MDJ1], 0.5% SDS, and 1 mg/ml proteinase K was added and incubate for 15 min at 37 °C. See this article for detail.Article Crystal Structure of the FeS Cluster–Containing Nucleotide E...
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