Folate receptor Positive or Negative ?

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Melting curve! What the factors that may affect on melting curve?

Hello every body. Using (SensiFast Sybr No-ROX one step kit) from Bioline, I did qPCR experiment used one -same- sample with 3 different concentrations of RNA and two different reference genes....

07 August 2016 2,428 6 View

RNA integrity?

I isolated total RNA from cancer cell lines using Trizol method.I wanted to test the integrity using bleach gel. as in the attached image the results were not cheerful to me. Can you please...

07 August 2016 9,732 7 View

Is it acceptable to do wounds healing experiments on cancer cell line?

I have met some one told me that he did some experiments on wounds healing using cancer cell line instead of normal cell line. I it applicable to do that and to publish the results?

10 November 2013 4,500 11 View

Discussion case ! Different Rf on TLC with the same Rt on HPLC ? is it one compound or two compounds ?

Two compounds have been isolated from the same fraction of a plant. On TLC they showed to have different Rf. For the physical characteristics, one of them is close to being crystal-like, while the...

04 May 2013 9,481 5 View

Confused about results of thin layer chromatography (TLC).

I have these results of TLC, I am not sure why this happens or whether the products are the same or not? Please see the picture attached bellow. This TLC was stained by vanilin and this is the...

01 February 2013 7,591 22 View

How to check Asiaticoside by TLC ?

I have a standard of Asiaticoside 5mg/10ml methanol. I need to check it on TLC. I have used mobile phase chloroform/glacial acetic acid/ methanol/ water 60/32/12/8 and 100/40/16/8 and also I used...

01 February 2013 2,566 12 View

How to check if crystals are organic or inorganic compounds?

I have some crystals with different shapes and some are cube like. I have run a methanolic extract of Centella asiatica in a silica column and one of the fractions, after a long time, showed some...

31 December 2012 9,309 50 View

Can I use GC MS to analyse Asiaticoside? What is the best condition ?

I am working on isolation of Asiaticoside (molecular weight is 959.12) from the Centella asiatica plant. I got methanolic extract after applying the grounded leaves to Soxhelt, then I have applied...

31 December 2012 8,386 5 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Saleh Alkarim

Hello

see links here will help you

Good Luck

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4376802/

http://www.ncbi.nlm.nih.gov/pubmed/23751075

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521101/

http://www.morphotek.com/publications-(1)/O-Shannessy-et-al---Oncotarget-2011---Characteriza.aspx

Helmi Y. Alfarra

Thank you @Saleh Alkarim

I was wondering if there is a data base that give an idea about the cell lines and the if there is a list of the cell lines that express FR ? to build my hypothesis according to that

Shadi Tarazi

If you brought cell line from ATCC you will find all information about characteristic on the web site