RNA integrity?

I isolated total RNA from cancer cell lines using Trizol method.I wanted to test the integrity using bleach gel. as in the attached image the results were not cheerful to me. Can you please...

07 August 2016 9,610 7 View

Melting curve! What the factors that may affect on melting curve?

07 August 2016 2,304 6 View

Is it acceptable to do wounds healing experiments on cancer cell line?

I have met some one told me that he did some experiments on wounds healing using cancer cell line instead of normal cell line. I it applicable to do that and to publish the results?

10 November 2013 4,381 11 View

Discussion case ! Different Rf on TLC with the same Rt on HPLC ? is it one compound or two compounds ?

Two compounds have been isolated from the same fraction of a plant. On TLC they showed to have different Rf. For the physical characteristics, one of them is close to being crystal-like, while the...

04 May 2013 9,347 5 View

Confused about results of thin layer chromatography (TLC).

I have these results of TLC, I am not sure why this happens or whether the products are the same or not? Please see the picture attached bellow. This TLC was stained by vanilin and this is the...

01 February 2013 7,414 22 View

How to check Asiaticoside by TLC ?

I have a standard of Asiaticoside 5mg/10ml methanol. I need to check it on TLC. I have used mobile phase chloroform/glacial acetic acid/ methanol/ water 60/32/12/8 and 100/40/16/8 and also I used...

01 February 2013 2,446 12 View

How to check if crystals are organic or inorganic compounds?

I have some crystals with different shapes and some are cube like. I have run a methanolic extract of Centella asiatica in a silica column and one of the fractions, after a long time, showed some...

31 December 2012 9,102 50 View

Can I use GC MS to analyse Asiaticoside? What is the best condition ?

I am working on isolation of Asiaticoside (molecular weight is 959.12) from the Centella asiatica plant. I got methanolic extract after applying the grounded leaves to Soxhelt, then I have applied...

31 December 2012 8,261 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View