Hi, I am synthesizing pentapeptide (GGGYK-Biotin) by FMOC SPPS using Rink amide resin. GGGY is a linker and K-biotin is a label. Unlike other fmoc amino acids, biotin-lysine did not get dissolve in DMF and hence I use NMP as a solvent. Would it make any change in the synthesis?
Also, how can I do coupling for this peptide?