Hi all scientists,
I am going to determine the LIVE/ DEAD fungi and bacteria cells with fluorescent dye in the possible biofilm on my plastic films surface.
I have TTC (tetrazolium chloride) but I do not think this is fluorescent. I am going to order dyes and prepare it by myself ( viability kits are very expensive). Does anyone know what dye I should use to be suitable for both bacteria and fungi. Is the protocol and method. In my mind FDI (fluorescein diacetate) for staining membrane and Pi (propidium iodide) for staining nucleus.
Many thanks in advance
if anyone has done it before or have a nice protocol please share it with me.
Hello Navid! You may tray solvatochromic fluorescent membrane probes like NR12S or F2N12SM [methylated] (JACS 2010 and RSC Advances 2015)
Hope this can be helpful.
Hi Navid,
You can stain the bacteria with syto9 for live and PI for dead assay. based on method you want to use, protocol may change. I have used LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit, for flow cytometry which worked fine for me. Also, it has a protocols for preparing samples.
Hope this will help you.
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes