Can anybody suggest a free web-based induced-fit docking tool?

I am looking for a free web based docking tool that takes into account backbone protein flexibility via induced-fit upon ligand docking?

01 February 2019 7,439 8 View

How to save/export a 3D SDF file using Open Babel?

I want to convert a SMILES code into a 3D SDF and PDB files to use for docking.I am using the following module: http://www.cheminfo.org/Chemistry/Cheminformatics/FormatConverter/index.html The...

01 February 2019 7,418 5 View

What are the kcat values of yeast hexokinase (Saccharomyces cerevisiae) for both glucose and ATP?

I am looking for kcat values (glucose and ATP) of hexokinase enzyme from common bakers yeast under physiological conditions (~pH 8, ~ 25 Deg C) along with reference preferably using...

06 July 2018 8,262 4 View

What are dimensions of ortho-silicic acid?

I need the exact diameter of ortho silicic acid, Si(OH)4. Kindly provide reference if possible. Thanking in advance for help.

04 May 2018 3,480 5 View

Is there any biological role (significance) of FRET from Trp residues to heme in peroxidases such as in Horseradish peroxidase?

Although HRP lives under darkness most of its natural life, what possible biological role Energy transfer from Trp to heme can have? Can it possibly effect Fe reactivity in Compound I or II if...

05 June 2015 4,665 3 View

Is there a bioinformatic tool to determine hydrphobic core amino acids by inputing a PDB file?

I want to determine which amino acids in a Protein structure are involved in Hydrophobic core. Is there a simple tool which requires a PDB file as input?

09 October 2014 1,098 5 View

What to do if you realize after submission to an open-access journal that it has bad reputation?

07 August 2014 9,881 2 View

How can I construct a Calcium-Green Standard curve without using commercial kit?

I want to construct a calcium standard curve using Calcium-Green dye and my own 1-39 uM calcium chloride solution. Is it possible to construct a good standard curve without using the commercial...

08 September 2013 3,001 3 View

What is the recipe for complete protein hydrolysis using nitric acid?

I want to completely hydrolyze a peroxidase enzyme with NITRIC ACID to determine Calcium content by ICP. Can anybody tell me a tried and tested simple recipe for complete protein hydrolysis...

07 August 2013 7,619 5 View

What is a positive peak at 305 nm in a purified protein using Near-UV CD show?

The protein has 3 Tyr and 3 Phe and 0 Trp residues. It show a sharp peak at 365 nm corresponding to Phe and 3 peaks between 272 and 285 nm corresponding to Tyr but I am wondering what a positive...

04 May 2013 5,905 3 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 7,968 5 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

How strong is Flag tag binding to Anti-Flag antibody?

I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...

11 December 2019 2,406 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,608 1 View

Can I restrict the amount of one species when using KinTek for kinetic fitting?

I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...

09 October 2019 3,835 4 View

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me. Does somebody have this plasmid?

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...

05 June 2019 4,133 1 View

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08 January 2019 3,512 5 View

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31 December 2018 8,083 5 View