Dear Fatma Abdel Wahab,

The difference is that FCS is an internationally agreed file format (which includes all settings of your equipment) to store FCM data with the possibility to rerun the experiment, whereas LMD is a listmode, ie it describes the data in a linear way (time domain). A suitable software package, free for non-commercial use, is Cyflogic. http://www.cyflogic.com/ This software can read .FCS files and gives you lots of opportunities to visulalise and process your data. I have used it for several publications, It produces nice graphs of high quality. I hope this is helpful. Kind regards, Harrie Verhoeven.

Thanks Harrie