Hello specialist in stomatas,
I would like to analyze the stomata at various stage of ABA and/or flg treatments. So I was wondering if its possible to fix the leaf samples and then analyze them later, so as to minimize the errors.
Thanks.
Hi Anamika, have you tried to fix them in ethanol and acetic acid solution?
Hi, Absolutely you can make imprint with nail polish, then fixed, and analyzed when you have spare time. For example, in paper http://dx.doi.org/10.12657/denbio.073.017 is good described methodology. Also, the leaf gas exchange parameters can be strong correlated to your experiment. But take a care, all three parameters are extremely plasticity to abiotic and biotic factors, therefore you must absolutely conduct sampling properly, and micro-meteorological data can very useful.
Thanks all of you for your suggestions.
Hi Olga Bykova. I tired to fix once, but somehow it didnt worked for me, and also I am not sure if the apperture of stomatal pore will be affected after fixing in ethanol and acetic acid!
@Andrew and @Sasa, do you think the imprints can be useful for apperture measurements? I was wondering as we leave the nail varnish on leaves to dry out for atleast 5-15 mins, this might affect stomatal pore itself!
Hi Anamika Rawat ,
Absolutely yes. This method is generally accepted and used to measure stomal dimensions and it's density. Therefore, in the future, when you submit paper to the journal, the editorial board as well as reviewers will accept this method as suitable. Please, follow the methodology compleatly.
ps. The nail varnish is dry from less then one minute, during the hot and sunny days. If you have questions, ask me, I will be happy to help if I know.
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