Hi,
I've extracted RNA from fixed cells, but ratios measured with nanodrop are low : 260/280 = 1,7 and 230/260 = 1,35 !
I used trizol, and I added two phenol/chloroform steps. Then, the ethanol steps (100% ethanol for precipitation and two washing with 70% ethanol )... When I don't fix cells, ratios are perfect...
Have Anyone a protocol to purify rna of fixed cells ?
Thanks !