Hi, I'm doing immunofluorescence in thick agarose sections (100 microns), but with confocal microscopy I got only 40 sections of 1 micron (z-stack).

I tested to put my slice between a slide and a coverslip but I thought that my slice was crushed. So I tried without coverslip but result is the same. How do you do to observe yours sections in microscopy ? Thanks !

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