Dear all,
Acriflavine agglutination test is being used as a common biochemical test to differentiate smooth and rough strains of bacteria such as Brucella spp and Salmonella spp. What is the mode of action of this reaction? If anyone can give a biochemical explanation, it would be highly appreciated. What acriflavine dose in the reaction? With what cellular components does it react?
Thank you,
Amal.
- Why is agglutination a sensitive method for antibody detection?
Agglutination is a sensitive method for the detection of antibody because much less antibody is required to agglutinate particles containing antigenic patches on their surfaces than is needed to aggregate antigens in free solution. This chapter provides an overview of agglutination and flocculation. Agglutination and Flocculation - ScienceDirect
https://biologyreader.com/agglutination-reaction.html
- How is agglutination performed to type? red blood cells?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959259
- Which is more sensitive Prozone or agglutination?
How is agglutination related to the prozone phenomenon?
Therefore, agglutination reactions are the reaction between antibody and antigen that result in visible clumping. These antibodies are what we call as “agglutinins”. More importantly, an excess of antibody inhibits the agglutination reaction. Thus, we call this inhibition as “prozone phenomenon”.
- Agglutination and Flocculation - ScienceDirect
- https://www.sciencedirect.com/science/article/abs/pii/S0300908403000427
Dear Dr. Len Leonid Mizrah,
Thank you very much for your detailed answer. It is definitely helpful.
Amal.
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