Hi everyone!
I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or protocol.
Thanks in advance
Habibi, how about this one?
Article Using formalin fixed paraffin embedded tissue to characteriz...
QIAamp DNA FFPE Tissue Kit
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/qiaamp-dna-ffpe-tissue-kit/#orderinginformation
Dear Izzati San!
Finally, I ordered the below kit.
https://www.qiagen.com/jp/shop/pcr/qiaamp-ucp-pathogen-mini-kit/#orderinginformation
The reference article is:
"Racsa LD, DeLeon-Carnes M, Hiskey M, Guarner J. Identification of bacterial pathogens from formalin-fixed, paraffin-embedded tissues by using 16S sequencing:retrospective correlation of results to clinicians responses. Hum Pathol. 2017 Jan;59:132-138. doi: 10.1016/j.humpath.2016.09.015."
I tried to order this kit "https://www.bioquote.com/product/gsync%C2%99-dna-extraction-kit/".
The reference article is: " Hosein, H. I., EL-Nahass, E. L. S., Rouby, S. R., & El-Nesr, K. A. (2018). Detection of Brucella in tissues and in formalin-fixed paraffin-embedded (FFPE) specimens using PCR. Adv. Anim. Vet. Sci, 6(2), 55-62. ".
But could not order this kit from Japan. Therefore, I ordered the first kit.
By the way, Thank you for your recommendation.
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