I have a minimal medium where casein sodium salt needs to be added. As it's a medium to grow bacteria and to avoid contamination I have been trying to prepare a solution separately and filter sterilize it before adding to my media. The main problem is with 0,45 and 0,22 filters barely 2 mL can pass before the filter clogs up. anyone having the same experience? how do you filter sterilize it or do you just directly add the powder.

More Jennifer Moussa's questions See All
Similar questions and discussions