Filling western blot SDS gel empty wells can improve results?

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Shamsher S. Kanwar

If you keep some of the wells in the gel empty, it hall have no effect on the SDS-PAGE. Why are you worried about he empty wells?

Lady López

Empty wells will not improve the run of your gels. However, it's important to fill them (if you have many) so the run is not altered or distorted.

You can fill your empty wells with an equal volume of Loading buffer 1X.