Is it important to fill empty wells of SDS gel while running western blot? What can we load in empty wells?
If you keep some of the wells in the gel empty, it hall have no effect on the SDS-PAGE. Why are you worried about he empty wells?
Yes i do agree with Shamsher S. Kanwar sir.
Empty wells will not improve the run of your gels. However, it's important to fill them (if you have many) so the run is not altered or distorted.
You can fill your empty wells with an equal volume of Loading buffer 1X.
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