Filling western blot SDS gel empty wells can improve results?

More Iqra .N's questions See All

Subculturing of colletotrichum capsici is not able to do please help me?

Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...

17 July 2024 1,610 2 View

What is the minimun number of studies for each subgroup analysis in meta-analysis?

Hello everyone, I am conducting a meta-analysis to find if x has superior effect than y on the outcome. I used the SMD method and I got a significant moderate heterogeneity of a value I2:...

09 June 2024 8,446 3 View

Effect size to check if x is superior than y... How to calculate it? within or between?

Hello everyone, I am conducting a systematic review to see if treatment x is superior in its effect than treatment y. Since most the studies I found did not report interaction effect, I was...

24 May 2024 6,130 2 View

Are You Interested in Collaborating on Chemistry Research?

Hello ResearchGate Community, I am an MS Chemistry student with a keen interest in advancing my research skills and contributing to impactful projects. As I embark on my journey in the world of...

17 May 2024 3,213 4 View

What are recent UWB Antennas ?

I require recent UWB antennas manufactured

15 May 2024 3,888 1 View

Which is the best free software for RAPD data analysis?

I am searching for a free best software for RAPD data analysis and phylogenetic tree construction

09 May 2024 7,017 3 View

Is there a way to combine CFA with linear mixed model taking into account random effects?

I try to explaim better. I have the scores for 10 items. Each item score are decided between the accordance of two persons (say, e.g., a therapist and a patient). Therefore, there is much more...

24 April 2024 4,563 6 View

How do I interpret Hedgs g sign? How do I calculate effect size when only median with CI is reported ?

Hello everyone, I am writing a systematic review about if X experimental group has superior effect than Y comparison group on outcome. Please note that the lower the scores of the outcome, the...

19 April 2024 1,812 1 View

Methods to calculate Shannon Index?

I have three field margin types (grassy/shrubby/tree) and each type have 5 sites. I need to calculate Shannon index for insect counts from each field margin type.... Q1. In my case how should I...

19 April 2024 681 6 View

How to fix the error during importing Gas Phase reaction, surface reactiona and thermodynamic data Chemkin file in fluent?

Hello All, Greetings The issue I am encountering pertains to the Chemkin files to be uploaded into the Ansys Fluent software. Unfortunately, I am encountering persistent errors, and it seems the...

04 April 2024 8,189 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Shamsher S. Kanwar

If you keep some of the wells in the gel empty, it hall have no effect on the SDS-PAGE. Why are you worried about he empty wells?

Nagesh Kishan Panchal

Yes i do agree with Shamsher S. Kanwar sir.

Lady López

Empty wells will not improve the run of your gels. However, it's important to fill them (if you have many) so the run is not altered or distorted.

You can fill your empty wells with an equal volume of Loading buffer 1X.