Hello fellow researchers.
I am confronted to a filamentous fungi subculture problem:
Because of the lockdown, I could not properly store my filamentous fungi. When I got back to work, I could subculture most of them, however, I couldn't get some others to grow back, despite the fact the the medium has not dried out and even though I am using the same medium I used to cultivate them on before. I tried other media, supplemented with Yeast Extract/Malt extact, in vain. I tried to grow them on liquid medium, in vain.
Can you give some advice on how to grow them back?
Thank you very much.
Hi Takhi Djalila ,
For how long they have been in your medium (is it PDA?) during the lockdown? some fungal isolates remain to live on PDA plates for nearly a year, especially the spore-forming ones while some other filamentous fungi lose their viability within 3-4 months, especially the non-sporulating ones. Nutrient limitation and toxic metabolites accumulation could be the reasons. Try inoculating them into a potato dextrose broth or the broth medium you are usually using to grow your cultures. Incubate them at 28-30 C for few days with continuous shaking at about 100 rpm. If they are not still growing, I am sorry to say this but your cultures might have died. You have to isolate them again.
Phil Geis and Sandhya Jayasekara thank you very much.
I will try broth cultures. I hope I can recover them.
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