to culture E14 mouse cortex Neural Stem Cells, is it efficient to only add EGF to the culture media? or it is a must to add both EGF and FGF2 growth factors? also if adding FGF2, is it necessary to add heparin as well? how does heparin affect FGF2 action in culture?
Saleh Alkarim
Hello
Kindly look at links here
May help you
Good Luck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3577833/
http://dev.biologists.org/content/develop/131/14/3423.full.pdf
http://www.scielo.br/pdf/aabc/v81n3/v81n3a09.pdf
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Piergiorgio La Rosa
Are you culturing NSC in floating or adherent condition? Heparin is always necessary if you grow NSCs as neurospheres. In floating condition, if you use FGF2/EGF you will obtain better results in NSC cultures.
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