Looking for pharmacologist interested in collaboration in a project "tubulin poly. inhibitors/stabilizers?

If anyone interested in collaborative work "design, synthesis and biological evaluation of small organic compounds with potential cytotoxic and tubulin polymerization inhibitory activity? in a...

11 March 2020 9,533 3 View

Absolute bacterial cell enumeration using flow cytometry: freezing/preserving samples?

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03 February 2019 7,342 3 View

Importers of medical shoes in Belgium?

I am working on a project and need to identify possible importers of medical shoes - orthopeadic and diabetic in Belgium. The potential exporter is from Croatia.

02 April 2018 1,421 1 View

What is the cause of failure of detection of significant quantitive trait loci ?

excuse me for that as recently joined this field and trying to make my way. I have a data set of 1545 SNP markers from NGS for many traits of 63 individuals (F2 intercross) the genotypes analyzed...

28 March 2018 7,913 4 View

How to motivate company managers for higher participation in online research?

I am constantly bumping into research barrier of extremely low response rates to online questionnaires addressed to companies:only 5-6%. I am looking for your experience and advice on how to...

23 November 2017 9,522 6 View

How to induce an epigenetic modification?

05 September 2016 9,919 3 View

What is the best method to get rid of dicyclohexyl urea?

the by product generated upon using DCC

20 March 2015 4,104 2 View

Can anyone help with Poly-L-lysine?

I'm trying to repeat the experiment from 1977 - isolation of platelet plasma membranes on Poly-L-lysine coated Biogel beads. The authors don't mention the detail in which form was the...

15 December 2013 6,039 3 View

Marketing ethics in contemporary society

What do you think are the major issuea in marketing ethics in contwmporary society. Does makreting ethics efficiently responds to technological and other challenges?

01 January 1970 9,268 17 View

Barriers to innovativeness

What are major barriers to innovativeness, especially for SMEs? Are exporting companies more injovarive than local and why?

01 January 1970 9,263 46 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View