Dear Friends,
A great portion of the essential oil stuck to the wall of the clevenger while hydro-distilling German chamomile. What can we do to avoid the oil from sticking to the walls.
Thanks.
Hello there. We have encountered a problem when using salysilic acid in the cream formulations; I feel like sand grains in the product when salysilic acid is used in the formulation. Do you have...
09 October 2018 8,080 0 View
Hello there, I've got a problem guys! The status date of my manuscript changed to a new date, but the status has remained Under Review! What does this mean? Thanks.
07 August 2018 3,232 2 View
Hello there, We are trying to make Ag nanoparticles using plants extracts; what kind of extract either alcoholic or water extract is more suitable for making nanoparticles? Searching in the...
03 April 2018 185 4 View
Hello there. I need a method of alkaloids extraction from bulbs of Sternbergia. Do you know a general method of extraction that works with small amounts of plant material? Thanks in advance.
03 April 2018 5,133 8 View
Something for beginners would be the best.
07 August 2017 253 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
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I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
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I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
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I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View