Hi,
Does anybody have a good protocol for efficient extraction of DNA from muscle tissue (mouse)? Our standard lab protocol doesn't seem to be robust enough for muscle samples.
Thanks in advance,
Georgia
I am wanting to switch from self-cast to pre-cast gels for my EMSAs. I am currently trying to decide whether to buy a constant percentage gel (eg. 6%) native gel or whether to try a 4-16% gel for...
03 March 2024 7,959 0 View
I have tried purifying a fluorescently-labelled (Alexa647) PCR product from agarose gel using the Macherey Nagel Nucleospin kit but even after several elution attempts (reapplying the eluted...
05 October 2023 9,769 3 View
I am running an EMSA with a myogenic TF (myogenin with N-terminal GST tag expressed in BL21 E coli) and its wildtype binding site (labelled with Alexa647 for visualisation). I use 100 fmol...
30 October 2022 8,256 1 View
I am attempting to perform an EMSA with a transcription factor and its wildtype binding sequence but the first attempt showed that the protein never left the well. After some research, I have...
10 February 2022 8,404 4 View
Hello, I have consistently had problems with a variety of antibodies for p53 in Western blots (bands at incorrect heights / unspecific bands). I am trying to detect the level of p53 protein from...
14 February 2018 7,418 10 View
01 February 2018 2,361 11 View
I am reading through literature where they have used rat intestinal acetone powder in in vitro studies to look at alpha-glucosidase activity. I'm not sure how it works and can't find the...
04 May 2015 429 0 View
We only have a 100mM stock of bezafibrate. I read in literature that commonly used concentrations for treating human or mouse fat cells is between 100 and 500. Has anybody had experience using...
08 September 2014 4,333 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Are there any references that report the maximum isometric forces of shoulder muscles?
08 August 2024 6,060 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View