Extraction of DNA from muscle tissue (mouse)?

Hi, Does anybody have a good protocol for efficient extraction of DNA from muscle tissue (mouse)? Our standard lab protocol doesn't seem to be robust enough for muscle samples. Thanks in...

10 November 2018 8,958 0 View

Does anybody know of a p53 antibody that works well in Western blot for mouse protein?

Hello, I have consistently had problems with a variety of antibodies for p53 in Western blots (bands at incorrect heights / unspecific bands). I am trying to detect the level of p53 protein from...

14 February 2018 7,197 10 View

Does anybody know of a p53 antibody that works well in Western blot for mouse protein?

Hello, I have consistently had problems with a variety of antibodies for p53 in Western blots (bands at incorrect heights / unspecific bands). I am trying to detect the level of p53 protein from...

01 February 2018 2,244 11 View

What is rat intestinal acetone powder and how does it work?

I am reading through literature where they have used rat intestinal acetone powder in in vitro studies to look at alpha-glucosidase activity. I'm not sure how it works and can't find the...

04 May 2015 318 0 View

What are the best concentrations to use for treating T37i cells with bezafibrate?

We only have a 100mM stock of bezafibrate. I read in literature that commonly used concentrations for treating human or mouse fat cells is between 100 and 500. Has anybody had experience using...

08 September 2014 4,253 2 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

How do I analyse a Photoluminescence Spectrum ?

I have a few questions related to PL (images attached for same material by 2 methods), It would be great to get some help. 1. What is the physical meaning of highly intense PL? Does this mean...

02 February 2021 1,791 3 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View