I am reading through literature where they have used rat intestinal acetone powder in in vitro studies to look at alpha-glucosidase activity. I'm not sure how it works and can't find the description online.
I am wanting to switch from self-cast to pre-cast gels for my EMSAs. I am currently trying to decide whether to buy a constant percentage gel (eg. 6%) native gel or whether to try a 4-16% gel for...
03 March 2024 7,959 0 View
I have tried purifying a fluorescently-labelled (Alexa647) PCR product from agarose gel using the Macherey Nagel Nucleospin kit but even after several elution attempts (reapplying the eluted...
05 October 2023 9,769 3 View
I am running an EMSA with a myogenic TF (myogenin with N-terminal GST tag expressed in BL21 E coli) and its wildtype binding site (labelled with Alexa647 for visualisation). I use 100 fmol...
30 October 2022 8,256 1 View
I am attempting to perform an EMSA with a transcription factor and its wildtype binding sequence but the first attempt showed that the protein never left the well. After some research, I have...
10 February 2022 8,404 4 View
Hi, Does anybody have a good protocol for efficient extraction of DNA from muscle tissue (mouse)? Our standard lab protocol doesn't seem to be robust enough for muscle samples. Thanks in...
10 November 2018 9,087 0 View
Hello, I have consistently had problems with a variety of antibodies for p53 in Western blots (bands at incorrect heights / unspecific bands). I am trying to detect the level of p53 protein from...
14 February 2018 7,418 10 View
01 February 2018 2,361 11 View
We only have a 100mM stock of bezafibrate. I read in literature that commonly used concentrations for treating human or mouse fat cells is between 100 and 500. Has anybody had experience using...
08 September 2014 4,333 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...
01 August 2024 629 3 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View