I want to ask how to interpret the results of GC-MS?

i performed GC-MS of my plant extract samples now i want to know that how to find out the compounds from that data it is having peak area, Area %, peak height, retention time etc. How to use the...

31 December 2018 9,882 5 View

I have used 15 mM H2O2 to carry out the catalase assay from plant tissues . how can i calculate the EC of H2O2?

I have used 1.5 ml phosphate buffer and 0.1 ml enzyme extract.

11 December 2018 7,618 2 View

I carried out SOD assay finding problem in the calculations ?

here am attaching a file of readings ,Total rxn volume is 3 ml, enzyme volume is 0.1ml.

11 December 2018 6,751 6 View

Carried out catalse assay (OD/Time) finding problem in calculations?

i used 3 ml total Rxn volume ,15mM 30 % H2o2, 0.1 ml enzyme extract how to calculate Unit/ml FW? and how to present the data Attaching file of my readings

11 December 2018 6,900 3 View

Which statistical test can i use for Disc diffusion assay and MIC ?

I have performed the disc diffusion assay against the plant material extracted in four solvents (Methanol,Acetone, chloroform, pet ether) against six bacterial strains and i used four different...

05 June 2018 550 3 View

Green synthesis from the plant extracts?

I want to prepare Nanoparticals from the plant extracts (Family Asteraceae) so i want to know the criteria of selection for different metal Nanoparticals for my plant extracts. In some previous...

05 June 2018 9,857 15 View

How can i prepare the 100mM Potassium Phosphate buffer (pH-7.0 and 7.8)?

I am carrying out the antioxidant enzymatic activity of plant extracts for that i want pot phosphate buffer of pH- 7 and 7.8.

05 June 2018 8,435 5 View

Antioxidant enzyme activity?

I carried out SOD, GST and Catalase activity from the different plants of family Asteraceae in normal condition no stress nd all. Now i want to know should i use a standard(i.e Enzyme SOD and...

05 June 2018 7,776 15 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View