Extraction of Artemisin from Artemisia scoparia?

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I want to ask how to interpret the results of GC-MS?

i performed GC-MS of my plant extract samples now i want to know that how to find out the compounds from that data it is having peak area, Area %, peak height, retention time etc. How to use the...

31 December 2018 10,030 5 View

I have used 15 mM H2O2 to carry out the catalase assay from plant tissues . how can i calculate the EC of H2O2?

I have used 1.5 ml phosphate buffer and 0.1 ml enzyme extract.

11 December 2018 7,720 2 View

I carried out SOD assay finding problem in the calculations ?

here am attaching a file of readings ,Total rxn volume is 3 ml, enzyme volume is 0.1ml.

11 December 2018 6,839 6 View

Carried out catalse assay (OD/Time) finding problem in calculations?

i used 3 ml total Rxn volume ,15mM 30 % H2o2, 0.1 ml enzyme extract how to calculate Unit/ml FW? and how to present the data Attaching file of my readings

11 December 2018 6,997 3 View

Which statistical test can i use for Disc diffusion assay and MIC ?

I have performed the disc diffusion assay against the plant material extracted in four solvents (Methanol,Acetone, chloroform, pet ether) against six bacterial strains and i used four different...

05 June 2018 653 3 View

Green synthesis from the plant extracts?

I want to prepare Nanoparticals from the plant extracts (Family Asteraceae) so i want to know the criteria of selection for different metal Nanoparticals for my plant extracts. In some previous...

05 June 2018 9,968 15 View

How can i prepare the 100mM Potassium Phosphate buffer (pH-7.0 and 7.8)?

I am carrying out the antioxidant enzymatic activity of plant extracts for that i want pot phosphate buffer of pH- 7 and 7.8.

05 June 2018 8,536 5 View

Antioxidant enzyme activity?

I carried out SOD, GST and Catalase activity from the different plants of family Asteraceae in normal condition no stress nd all. Now i want to know should i use a standard(i.e Enzyme SOD and...

05 June 2018 7,871 15 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How can I extract my bibliography from researchgate ?

how can I extract my bibliography from researchgate ?

28 July 2024 6,737 1 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View