Extraction of Artemisin from Artemisia scoparia?

I I want to extract Artemisin from Artemisia scoparia can anyone help me how can i extract out the artemisin -A sesqueterpene lactones from this plant.

01 February 2020 9,297 2 View

I want to ask how to interpret the results of GC-MS?

i performed GC-MS of my plant extract samples now i want to know that how to find out the compounds from that data it is having peak area, Area %, peak height, retention time etc. How to use the...

31 December 2018 9,882 5 View

I have used 15 mM H2O2 to carry out the catalase assay from plant tissues . how can i calculate the EC of H2O2?

I have used 1.5 ml phosphate buffer and 0.1 ml enzyme extract.

11 December 2018 7,618 2 View

I carried out SOD assay finding problem in the calculations ?

here am attaching a file of readings ,Total rxn volume is 3 ml, enzyme volume is 0.1ml.

11 December 2018 6,751 6 View

Which statistical test can i use for Disc diffusion assay and MIC ?

I have performed the disc diffusion assay against the plant material extracted in four solvents (Methanol,Acetone, chloroform, pet ether) against six bacterial strains and i used four different...

05 June 2018 550 3 View

Green synthesis from the plant extracts?

I want to prepare Nanoparticals from the plant extracts (Family Asteraceae) so i want to know the criteria of selection for different metal Nanoparticals for my plant extracts. In some previous...

05 June 2018 9,857 15 View

How can i prepare the 100mM Potassium Phosphate buffer (pH-7.0 and 7.8)?

I am carrying out the antioxidant enzymatic activity of plant extracts for that i want pot phosphate buffer of pH- 7 and 7.8.

05 June 2018 8,435 5 View

Antioxidant enzyme activity?

I carried out SOD, GST and Catalase activity from the different plants of family Asteraceae in normal condition no stress nd all. Now i want to know should i use a standard(i.e Enzyme SOD and...

05 June 2018 7,776 15 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View