Hi
did anyone try to express a mouse protein in a human cell line?
I am investigating the role of a protein in the regulation of the tumor microenvironment (TME) in renal cell carcinoma. I have some interesting results in TME alterations by using murine renal carcinoma RENCA cells (overexpressing such a protein) when implanted in mice. I want to validate my data using a human cell line (i.e. 786-0 ), but unfortunately the human ortholog can not bind to the mouse receptor. So, when I implant the human cells (overexpressing the human ortholog) in mice, this protein is basically inactive in the murine microenvironment. I think that the expression of the mouse protein in the human cell line could be a valid alternative for confirming my preliminary data using another cell line (unfortunately, there are no available murine cell lines other than RENCA cells).
Thanks
Hi Andrea,
I expressed mouse genes in Human cell lines. HeLa and MDA-231. The vectors were viral and eukaryotic. I would approach the problem in three ways:
1. Change the expression vector.
2. Change human cell lines ( do try several ones).
3. Change the mouse sequence from mouse to human.
If 1 and 2 doesn't work pass to step 3. Otherwise is just of waste of experimental time.
Hope it works.
There are no big problems in expressing a mouse gene in a human cell line. This is done very often. Here are some example retrieved from a query in the Cellosaurus that search for human cell lines having a "MGI" Xref (ie a transfected or transduced mouse gene):
https://web.expasy.org/cgi-bin/cellosaurus/search?input=9606+MGI
Best regards
Amos Bairoch
Dear Dr. Emanuelli,
I agree with Dr. Bairoach.
Also I saw this related question and answer:
https://www.researchgate.net/post/Can-a-human-gene-be-expressed-in-a-mouse-cell-line-under-the-control-of-the-endogenous-human-promoter
Best Wishes!
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