Andrea Emanuelli

3 Questions 10 Answers 0 Followers

Questions related from Andrea Emanuelli

We see protein knockout by Western Blot analysis, but not by RT-PCR. How is possible?

In my lab we succeeded to knock out a gene using Crispr-cas9 technology. We assessed level of KO by western blot analysis (but KO is not 100%, it is around 70-80%). However, by RT-PCR analysis I...

15 January 2018 8,672 5 View

My Hematoxyilin stains too much and I can not see DAB brown staining. How can I regulate its intensity of staining in IHC?

Hello people I am using HEMATOXYLIN QS (H-3404) from Vector Laboratories for nuclear counterstaining of mice tissues. Unfortunately, in my case this ematoxylin is so strong that removes or covers...

24 November 2015 3,847 9 View

Expression of mouse gene/protein by human cell line

Hi did anyone try to express a mouse protein in a human cell line? I am investigating the role of a protein in the regulation of the tumor microenvironment (TME) in renal cell carcinoma. I have...

01 January 1970 9,730 3 View