Experience KO in the adult mice brain?

13 August 2018 1 6K Report

Hi all,

I have a validated sgRNA to KO our gene of interest in the adult mice brain And I was thinking of using Lenticrispr V2 with mcherry and to inject it ICV, has anyone had any experience with such a project? I couldnt find any relevant articles.

Do you think it would be better to just inject a virus with only the sgRNA into the brain of a cas9 KI mouse?

Thanks in advance :)

Amr Salem

Hi, Karmon

I hope you are well, I think trying to KO in adult mouse brain is a very difficult task since its non dividing cells, am not sure that your transduction efficiency will be 100% and your KO cells will be Homozygous.

SO, I think it will be better if you try dCas9 will give you considerable and reliable effects, transduction of your sgRNA targeting your interest gene promoter in dCas9 KI mice

Best of Luck

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