.
You can grow M.tuberculosis in Sauton's medium (Protien free)You will get good pellicle growth.
HI Vithal and thank you for your answer. But I need more details- can I grow TB in shaker at 37 or does it need special conditions of growth?
If you shake incubate at 37 yield may in suspension, but the media suggested is generally used for pellical growth.you may incubate the containers at 37 in 6% CO2 atmosphere.
Hi all! I have performed a behavioral test (visual cliff) and i want to classify the mouse location in 3 different parts of the arena, I want to be able to extract cumulative time, latency to...
23 January 2020 4,111 2 View
Hi all, I have a set of open field test in which i want to detect grooming, supported rearing and unsupported rearing behavior. Ethovision XT was somewhat OK for the grooming but couldn't...
23 January 2020 9,011 1 View
Hi all, I have a validated sgRNA to KO our gene of interest in the adult mice brain And I was thinking of using Lenticrispr V2 with mcherry and to inject it ICV, has anyone had any experience with...
12 August 2018 5,658 1 View
I need to design an sgRNA that contains TTTT thus making expression with RNA pol III impossible. It will need to be expressed in mammalian cells. Any help will be extremely welcome (please refer...
28 January 2018 4,204 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Roflumilast Cream Improves Signs and Symptoms of Plaque Psor...
29 July 2024 5,250 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View