Can you express sgRNA with a none U6/ U3 promoter in an easily cloned vector?

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How to manually score a behavioral test for free?

Hi all! I have performed a behavioral test (visual cliff) and i want to classify the mouse location in 3 different parts of the arena, I want to be able to extract cumulative time, latency to...

23 January 2020 4,111 2 View

Automated software for grooming and rearing behavior in mice?

Hi all, I have a set of open field test in which i want to detect grooming, supported rearing and unsupported rearing behavior. Ethovision XT was somewhat OK for the grooming but couldn't...

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Experience KO in the adult mice brain?

Hi all, I have a validated sgRNA to KO our gene of interest in the adult mice brain And I was thinking of using Lenticrispr V2 with mcherry and to inject it ICV, has anyone had any experience with...

12 August 2018 5,658 1 View

I would really appreciate it if someone can please send me someone protocols for growing M. tuberculosis in large amounts (10 - 50 Liter)?

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Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

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How to confirm the site-directed mutagenesis result without performing NGS?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

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E.coli contamination in human RNA seq data ?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

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Gidi Karmon

Thanks! I will try it. Still, if anyone has more ideas ( just in case) I would greatly appreciate it!

Di Xia

Hi, Gidi, I am facing the same issue. One possibility is to do sgRNA IVT or to use Chemical synthesised guide RNA. A few companies are making that. The best ones are from IDT (crRNA + tracrRNA) or Synthego (full length sgRNA).