Hello everyone,

have you ever created DNA libraries for RNA seq starting from Extracellular vescicoles? we are trying to investigate the RNA content of EVs derived from pancreatic cancer but we are observing strange profiles at the Tapestation. The quantity and concentration of the libraries seem fine to do RNA seq with illumina sequencer but instead of the conventional profile at the tapestation we observe manhy peaks. Do you believe it could be a problem?