Giulia Lencioni

11 Questions 15 Answers 0 Followers

Questions related from Giulia Lencioni

Contaminant cells in pancreatic cancer patient derived organoids?

Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...

16 July 2024 724 3 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

07 June 2024 5,559 4 View

Pancreatic cancer organoids not growing well?

Hello everyone, I am currently working on patient derived organoids from pancreatic cancer (PDAC) patients. I noted that my organoids never grow too much. after 1 month in culture they are...

05 June 2024 572 7 View

Does anybody can help me understand how to co-cultivate platelets and cells?

Hello everyone, I need help to understand how I can "co-cultivate" human platelets (derived from whole blood) with cancer cells. I have specific questions: -How can I avoid platelets from...

22 April 2024 7,438 1 View

How to keep alive Platelets in vitro?

Hello everyone, I would like to use platelets in a co-culture with other cells. I was wondering which are the conditions to keep alive the platelets: -since I am going to buy my platelets, I was...

15 December 2023 2,302 10 View

Better way to co-cultivate pancreatic cancer organoids and CAFs?

Hello everyone, I started cultivating pancreatic cancer organoids obtained from patient-derived tissues. I would like to establish co-cultures with the CAFs (I have them) derived from the same...

23 November 2023 1,819 0 View

How to extract RNA from OCT (optimal cutting temperature) clinical samples?

Hi everyone. I would like to know if extracting RNA from OCT-embedded tissues is possible. Would you happen to have any recommendations? Can you extract RNA with a high purity level? In...

29 September 2023 3,755 0 View

How to reconstitute the collagenase IV from Clostridium histolyticum ?

Hi everyone, I have a doubt about the buffer needed to resuspend Clostridium histolyticum collagenase IV (the one I'm using is C5138 from Sigma). Should I use DPBS with or without calcium and...

21 August 2023 3,588 0 View

How to cryopreserved human tissue samples?

Hello everyone. I would like to know if there are some contraindications in moving human tissue samples stored for more than 6 months at -80°C (with medium and DMSO), to a new temperature of...

02 August 2023 5,340 2 View

How do I calcluate the Wnt activity using the Dual luciferase assay (PROMEGA)?

Hi everyone, I'm using a dual glo luciferase assay by promega to assess Wnt activity in my Wnt conditioned media. The setup in my protocol requires to use -a negative control (NEG, non responsive...

24 July 2023 3,477 4 View

What is the role of FBS in Rspo1 conditioned media production?

Hi, I'm following a protocol to produce Rspo1 conditioned media by using 293T cells. I was wondering why it is recommended to prepare the conditioned media by treating the cells with AdDMEM f12;1%...

18 July 2023 8,382 2 View