12 Questions 15 Answers 0 Followers
Questions related from Giulia Lencioni
Hello everyone, have you ever created DNA libraries for RNA seq starting from Extracellular vescicoles? we are trying to investigate the RNA content of EVs derived from pancreatic cancer but we...
11 June 2025 7,649 1 View
Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...
16 July 2024 883 3 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
07 June 2024 6,050 4 View
Hello everyone, I am currently working on patient derived organoids from pancreatic cancer (PDAC) patients. I noted that my organoids never grow too much. after 1 month in culture they are...
05 June 2024 737 7 View
Hello everyone, I need help to understand how I can "co-cultivate" human platelets (derived from whole blood) with cancer cells. I have specific questions: -How can I avoid platelets from...
22 April 2024 7,514 1 View
Hello everyone, I would like to use platelets in a co-culture with other cells. I was wondering which are the conditions to keep alive the platelets: -since I am going to buy my platelets, I was...
15 December 2023 2,384 10 View
Hello everyone, I started cultivating pancreatic cancer organoids obtained from patient-derived tissues. I would like to establish co-cultures with the CAFs (I have them) derived from the same...
23 November 2023 1,896 0 View
Hi everyone. I would like to know if extracting RNA from OCT-embedded tissues is possible. Would you happen to have any recommendations? Can you extract RNA with a high purity level? In...
29 September 2023 3,825 0 View
Hi everyone, I have a doubt about the buffer needed to resuspend Clostridium histolyticum collagenase IV (the one I'm using is C5138 from Sigma). Should I use DPBS with or without calcium and...
21 August 2023 3,666 0 View
Hello everyone. I would like to know if there are some contraindications in moving human tissue samples stored for more than 6 months at -80°C (with medium and DMSO), to a new temperature of...
02 August 2023 5,423 2 View
Hi everyone, I'm using a dual glo luciferase assay by promega to assess Wnt activity in my Wnt conditioned media. The setup in my protocol requires to use -a negative control (NEG, non responsive...
24 July 2023 3,561 4 View
Hi, I'm following a protocol to produce Rspo1 conditioned media by using 293T cells. I was wondering why it is recommended to prepare the conditioned media by treating the cells with AdDMEM f12;1%...
18 July 2023 8,452 2 View
