What is the accepted recovery rate range for an acid digestion(HCl:HNO3), followed by an AES analysis?

I'm doing a heavy metal analysis by digesting bird feathers using acids. And in order to verify whether the method i use and the instrument i use give accurate results i was planning to do a...

22 September 2020 4,129 3 View

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?. Is it 1.35? or is there some trick? I read that saturated NaCl soltion has specific gravity of 1.2. Also...

13 May 2020 5,065 2 View

How to avoid uneven (intensity) bands during Western Blotting?

I've uneven bands (size, intensity, and staining) when doing Western Blotting and I'm wondering what the problem could be. The protocol: -10% separating gel (0.375M Tris pH8.8; 0.1% SDS; 10%...

14 July 2019 6,577 8 View

How to ask a good question in RG? and get many responses?

Do we need to be specific? Sometime I feel that if we become specific with more details when we ask the questions, we loose some potential researchers from other fields. So the number of answers...

27 May 2019 3,627 24 View

Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

I would like to know the, Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

27 February 2019 9,882 6 View

Hello.. Is it possible to analyse the cross sectional area propagation of a flame by using mesh free method ?

18 January 2017 9,363 1 View

How to dissolve PEI (polyethylenimine)?

20 April 2016 2,880 22 View

Is there any possibility that tofu protein recovery can achieve more than 100%?

I make tofu from vegetable soybean (edamame). Water to bean ratio for tofu making is 1:12 and CaSO4.2H2O as coagulants. Vegetable soybean has moisture content 64% (wet basis) and protein 5% (wet...

16 October 2015 3,816 12 View

Could someone help me with some Neuronal Tissue Co-IP issues?

I have been doing co-immunoprecipitations for a while now using my own buffers (CHAPS lysis buffer + protease & phosphatase inhibitors) and Dynabeads protein G beads. Useful information: -The...

21 January 2014 5,209 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Does anyone know what are the pros and cons for using autoencoders instead of CNNs for features extraction in neural networks?

Are there papers that talk about the possible advantages and disadvantages of using autoencoders instead of CNNs for feature extraction in neural network?

15 February 2021 6,952 3 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View