I use GelRed for post staining of the gel. I am facing smearing problem as well as the lowest size band in the ladder always appears as very thick(shown in the picture). Is Ethidium bromide better than GelRed? If so, then why it is better?
Hallo Sadia,
Regarding your quesion, Ethidium bromide (EtBr) has the advantage of visulaising clearly bands of small sizes even in a small quantity.
Regarding your photo, it looks nice by the way. You can judge it from the DNA ladder that you used and it looks well. It is also clear that all of the other lanes (except the middle one) did not work well (there is a problem within the PCR reaction itself).
I do not think jumping from GelRed to EtBr would be helpful for you, instead you have to think about the stratgey of loading itself. How much of your samples did you use for loading your gel? what is the concentration of your gel itself? if possible, what is the concentration of your samples themselves?
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