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I got the desired band after pcr. I extracted DNA using boiling method. But few months later I am using the same DNA template and same pcr protocol is also maintained. But this time, I am not...
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I use GelRed for post staining of the gel. I am facing smearing problem as well as the lowest size band in the ladder always appears as very thick(shown in the picture). Is Ethidium bromide better...
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