I have cultured EPC from blood PBMCs. After growing out and achieving the cobblestone morphology/ positive test for VWF, another cell type is out-growing my ECs (In white). Can you tell me what this is?
FYO- cells are cultured in promocell endothelial media (2% FBS).
Dear Erica,
Many thanks for your answer. So from your experience, is this a problem that occurs intermittently rather than all the time? I'm a little confused over the origins of these contaminating cells; Is one to assume that they are derived from their own progenitor equivalents such as fibrocytes? All so ,rather odd that everyone seems to report that they do not 'show up' until a passage or two rather than from the onset.
kr
Charlie
Dear Erica,
Similar question, also after a month of culture, (no passage, still same flask from which PBMCs were plated), I often end up with cells like these? Have you seen these before? What is the latest you have waited to see EC colonies form?
many thanks
Charlie
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