Hello Charlotte Van Liedekerke

Pre-coat the inside of a centrifuge (15 mL) tube with 2.5% bovine serum albumin (BSA) solution by repeatedly filling the tube with 2.5% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) without Ca2+/Mg2+ and emptying the tube.

Similarly, for 1 mL pipette tip, dip the full length of the tip in 2.5% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) without Ca2+/Mg2+ and pipetting 1 mL of this solution up and down twice. You could try this method. Maybe it will help prevent the loss. You can also try it out with FBS if BSA does not work.

Best.

Dear Malcolm Nobre ,

Thank you very much for your reply.

We will try your suggestion of using 2,5% BSA and see if we can optimize our protocol.

Best, Charlotte

I'd suggest coating with something like pluronic F127 or polyHEMA to reduce adherence.