Hi, I'm working on bovine intestinal organoids. I processed 3 samples of organoids, Intestinal tissue as a positive control and stomach tissue as a negative control in qPCR. In the case of positive control (Intestinal tissue), there was no product even for housekeeping marker. While the total RNA quantity and quality (for cDNA synthesis) was much better than that extracted from organoids and stomach tissue. I wonder what is the problem and how to troubleshoot it? I would be much grateful for the help.

Thank you.