Around a million are available in literature, search any alkaloids's isolation from plants! Briefly use slightly acidic aqueous and organic solvent mix!

Use soxhlet or maceration method, as alkaloids are dissolved in polar organic solvents like Dimethyl Sulphoxide, methanol etc. and even in acidic double distilled water, you must use polar solvent to extract.

Regards, Bipul Das Chowdhury

Hope this review will help.

Hi Nazmul,

It depends what is your sample. This paper used a validated method to extract alkaloids from plant, soil and water.

Article Occurrence of pyrrolizidine alkaloids in ragwort plants, soi...

You have an overview of the method recovery and matrix effect.

I hope this helps!

Dear Sir, Thanks for information