ELISA or Western blot for zebrafish vitellogenin?

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Matthew Sobo

TECO has a few VTG ELISA kits

https://www.tecomedical.com/en/ecotoxicology/REACH-Cyprinid-Vitellogenin-ELISA-TECO

also a way to test for VTG from mucus instead of sacing the whole fish

https://www.tecomedical.com/en/ecotoxicology/Mucus-Collection-Set-TECO

Isabel Forner-Piquer

And regarding to quantify vitellogenin in the liver/ovary and not in the plasma? there is any recommended anti-body?

Isabel, as far as I am aware, the antibody is the same