Share a copy of RaFD Dataset?

I tried to get this dataset from the original link but no one approved my requests for weeks. Can someone share with me a private link to use? You can send it to my institutional email:...

14 February 2021 1,908 1 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

Aspen plus (hydrometallurgy set-up) & reactions solid-liquid ?

I have to simulate a hydrometallurgical process where there are the following phases: - leaching (solid-liquid reactions) - cementation (solid-liquid reactions) - electrowinning (energy ) -...

30 January 2021 9,225 3 View

Is there any programming code for the voltammetric simulation of modified composite electrodes?

Hi, I Would like to know if there is any programming code that anyone would like to share for a cyclic voltammetry simulation on a chemically modified composite electrode? Thank you!

17 January 2021 5,983 3 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

What is the cleanest / best way to handle different software dependencies / configurations?

Dear RG users, I noticed that several toolboxes developed for python are compatible with some specific releases and not with others and in general there is a precise set of tools defining the...

30 November 2020 4,545 5 View

Why maximum allowable limits for toxic metals or heavy metals in agricultural soils vary among countries and institutions?

For example, the maximum allowable limit for USEPA in agricultural soils are very low when compared to Tanzania's maximum allowable limits see figure below. Does this can affect the management...

23 November 2020 5,052 10 View

What is the best way to get organoids of the same size?

I would like to get organoids of the same size. We filter organoids using a 100 um filter and then pass that filtrate through a 40 um filter followed by flipping the 40 um filter. Then we wash the...

22 November 2020 9,391 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View