Hello,
I am a new research student and entirely new to the field and it's processes.
We wish to measure allopregnanolone in the blood via ELISA.
We anticipate we will get small and infrequent numbers of patients who volunteer for blood collection. Thus, it is essential we freeze the blood (to run them all on the same ELISA kit).
The blood will be frozen for no more than 1-3 months. It will be analysed once we reach a suitable number of samples. We have the capability to centrifuge the blood.
I have tried searching the literature on this matter but found relatively little. I suspect it is a matter of experience rather than anything someone puts in the method section of a paper. I would appreciate everyone sharing their experiences.
The samples maybe kept in storage for almost a month at -20 and less than 2 months at -80 degrees. Freeze thaw cycles are to be avoided which means that you can only thaw them once i.e. when you are about to run the ELISA.
Hope this helps.
hi
sample are serum,plasma,tissue homogenates or cell culture.
1. Samples should be used within 7 days when stored at 2-8℃, otherwise samples must be divided
and stored at -20℃ (≤1month) or -80℃ (≤6months) to avoid the loss of bioactivity and
contamination. Avoid repeated freeze-thaw cycles.
2. Please take the samples to room temperature (18-25℃) without extra heating before performing the assay
.and follow the attachments ,hope will be really useful.one is a ELISA kit insert and other one is ,UMEA University,swedon -published paper about ALLO.
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