Dear Hiral

You could start with gel filtration or you could concentrate your sample using centrifugal concentrators such of Amicon's, desalting step may help, all of the mentioned techniques are based on molecular weight differences, thus you should be able to get rid of polysaccharides.

Hope this helps   

Dear Hiral,

Instead of doing ammonium sulphate precipitation, you could perform three phase partitioning (TPP) to purify the protein. 

Dear Hiral,

you can use solvent like aceton or ethanol to concentrate your sample then using centrifuge ...good luck.

Hello,

What is your goal? purify protein in order to have some enzymic activity? If it is the case don't try to use aceton or ethanol, these reagents are using for denaturing the proteins.. By contrast the use of ammonium sulfate is a way for purification of active protein.