Either i have to wash my silver nanoparticles before taking uv-visible spectra or take uv-visible spectra with out washing of these particles?

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Ruptanu Banerjee

Yes it’s better to take both the readings, before and after. You may see some changes in the peak intensity and/or broadening of some peaks depending on your synthesis parameters.

Muhammad Ismail

Usually we took spectra before washing. After confirmation that our product is formed then we can washed them