Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

Do you think rote learning can be use as a strategy in teaching Writing skills?

I'm working on my research problem, looking into the role of rote learning in developing the writing skills of students.

22 December 2020 5,658 5 View

I need help for an experiment I propose. Can somebody recommend articles describing beam-splitters for charged particles?

An experiment that I propose contains a beam-splitter for protons. There is plenty of articles in the literature, about atoms interferometry. But, except for electron interferometry, I don't see...

22 November 2020 7,138 3 View

Is snap freezing method good to extract DNA from tissue samples?

I have to extract DNA from a small testis biopsy and we tried to homogenise the tissue with a 5mm bead in lysis buffer. However, the tissue remained intact. Is it better to do it when frozen in...

19 July 2020 2,809 2 View

Quantitative instruments to measure stigma related with employed adults with disabilities?

I am working on my dissertation for my Masters Degree. I am in need of a tested and reliable quantitative instrument that can help look at stigma related with employed adults with...

09 July 2020 1,890 6 View

Where can I obtain immortalized human sebocyte cell line SZ95?

Dear collegues, I am trying to obtain an immortalized cell line of human sebocytes and a come across the reference for SZ95. Is there a commercial vendor for this cell line? Thanks in advance!

15 March 2020 169 3 View

How to analyse cell proliferation data?

Dear all, I used the Click-it EdU flow cytometry assay to measure cell proliferation of my samples. However I do not know how to analyse them and interpret. How can I use the data to compare cell...

27 February 2020 5,861 1 View

How could I completely delete it?

I have two profiles. The first one was deleted a couple of months ago, however it still apperars on google whereby I search for my name. Moreover I am not able to log in with the date of the...

15 December 2019 8,217 3 View

How to measure enhancer activity?

I've seen some approaches such as using luciferase assay and ChIP-seq data, but I'm not sure which one to use.

27 November 2019 8,510 3 View

What theoretical background do you use to justify that appeal is determined by a number of celebrity attributes?

Hello! I am a student from Russia. Now i am working on my thesis on factor that affect income of models in fashion industry. It seems as you research experience on celebrity question somehow...

10 November 2019 2,393 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View