EGFR exon 20 mutated cell line

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Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

How can technological innovations be leveraged to overcome challenges such as unsuitable blood discards at regional blood transfusion center?

In a journal article written by Kavulavu et al. (2022), entitled “Challenges facing Blood Transfusion Services at a Regional Blood Transfusion Center in Western Kenya”, one of the key challenges...

11 April 2024 7,586 1 View

Can we perform Population genomics analysis with genome resequencing data?

Sometimes the problem in sequencing project formation hamper your analysis, therefore i want to ask the scientific community that if we can pool the samples of a population and do the genome...

08 April 2024 6,645 0 View

¿Como implementar un plan de negocios en una IPS DE SALUD MENTAL?

Necesito crear un plan de negocios en las áreas de estudio administrativo, técnico, ambiental, financiero y de mercadeo

18 March 2024 8,929 0 View

What is the relationship between use of Assistive technology and wellbeing in older persons?

Research Problem While evidence suggests AT can benefit older adults, a comprehensive understanding of its impact on their well-being remains unclear. To effectively support Older persons’...

20 February 2024 2,645 0 View

Which accelerometer is considered the best tool for measuring sedentary behavior for research purposes?

As a student researcher, choosing the right accelerometer for studying sedentary behavior requires careful consideration. The selected device should be sensitive to prolonged sitting and...

12 February 2024 5,274 1 View

Which accelerometer is considered most suitable for measuring physical activity intensities?

As a student researcher, choosing an accelerometer to measure physical activity intensities involves careful consideration of various factors. The selected accelerometer should demonstrate high...

12 February 2024 2,274 3 View

Is there a need to desalinate caulerpa racemosa specimen prior to extraction?

Hello! We are conducting an experiment on caulerpa racemosa crude methanolic extract and we are after the flavonoids present in the plant. According to some journals we read, they did not...

17 January 2024 5,280 0 View

How to write an abstract without conclusion?

I have a retrospective descriptive study, can I write an abstract without conclusion? And how it can be done? Thank you

10 December 2023 9,614 3 View

Cook et al., 2013 - How were epochs extracted and what is the prediction horizon?

While reading the work "Prediction of seizure likelihood with a long-term, implanted seizure advisory system in patients with drug-resistant epilepsy: A first-in-man study" I failed to figure out...

29 November 2023 8,283 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Philippe Fort

Hi Sofia,

You could contact people who submitted this poster.

Best,

Yang Huanhuan

Maybe you can contact the scientist at Creative-Bioarray.

https://www.creative-bioarray.com/

Shyam Nyati

Did you check ATCC? Something like

EGFR Genetic Alteration Cell Panel (ATCC® TCP-1027™)?

Did you contact any authors who published using Exon20 mutant cell-lines?

https://www.mycancergenome.org/content/disease/lung-cancer/egfr/64/

Good Luck,

Shyam