Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

What crossover operators can be used for a GA that finds suboptimal positions for a set of buildings?

I'm currently working on my undergraduate thesis where I develop a genetic algorithm that finds suboptimal 2D positions for a set of buildings. The solution representation is a vector of real...

11 January 2021 9,835 4 View

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

How did Rutherford and Thomson derive the equation for average deflection of a particle hitting an atom?

08 November 2020 9,046 2 View

Converting csv files with data for mapping rainfall on QGIS?

Hi, I am hoping to produce rainfall and temperature maps for a current project in QGIS. I have csv data downloaded which have been imported into QGIS. My aim is to be able to produce rainfall...

10 October 2020 4,930 5 View

How to control variables in a water hammer experiment?

I'm doing a high school experiment on how the impulse of water hammer is affected by pipe diameter, and I'm unsure of how to set up my experiment. My primary concern is safety and validity: - Is...

06 September 2020 1,252 3 View

Incorrect simulated conductance value compared to analytical solution using ANSYS Maxwell 2D?

31 May 2020 7,991 3 View

Reaction of hexamethyldisiloxane (HDMSO)+O2=SiO2+?

I'm giving a presentation on semiconductors and specifically the SiO2 insulating layer. The presentation is on kinetics so I kind of need to know the reaction. Problem is the papers I'm reading...

30 March 2020 8,643 3 View

Predicting the Age of home buyers - model based method?

All, I have a data-set which contains information on both the type of property an individual(s) purchased, along with some information on the individuals themselves. However, I am missing age...

19 February 2020 5,728 3 View

Are full-scale performance-based drama projects for adult EFL learners worthwhile?

Hello, I wonder if anyone would be kind enough to help with research for my dissertation concerning attitudes and approaches to full-scale stage or screen performance-based drama projects for...

27 January 2020 3,167 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View