Hi,
recently I am investigating the role of a harmful "Compound A" in the autophagy regulation of A549 cells. We found that Compound A significantly repressed the activity of cells in MTT assay. It also induced a strong autophagy. We observed a predominant increase in the levels of p62 and LC3B. After the pretreatment of late autophagy inhibitor, p62 and LC3B were further enhanced. Although from the results of MTT assay we know that this compound reduce the activity of cells (maybe by destroy the mitochondria of the cells), we did not see obvious phenomenon for the cell apoptosis by TUNEL assay.
I would like discuss a hypothesis for the mechanism of Compound A in A549 cells. Is it possible that, Compound A induced the damage of mitochondria. In the mean time, Compound A evoked the autophagy of cells to "eliminate" the damaged mitochondria of A549, thus exerted a protective effects in A549 cells? If yes, could any expert suggest reliable markers (except for cytochrome c)to examine the damage of mitochondria?
Isolation of mitochondria is an option in this study. However, we need an indicator of mitochondrial damage.
Many thanks,
CH
Not sure if you explored these options:
https://www.abcam.com/kits/mitochondrial-assays
https://www.detroitrandd.com/store/mitochondrial-dna-damage-copy-number-and-citrate-synthase-kits/mitochondrial-dna-damage-assay
Article Quantitative PCR-Based Measurement of Nuclear and Mitochondr...
Hi Sujana,
many thanks for your reply. It's really helpful. The mitochondrial DNA damage assay kit could be an excellent option to detect the damage of mitochondria.
With best regards,
CH
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