I did a Western Blot yesterday but, my band doesn't exist!
I think my antibody dilution is wrong... I used 1:10,000 for anti-ERG
What dilution should I try this time?
Hi Marian
It depends on which antibody you are using...
What does the datasheet of the antibody says? (For example abcam suggests for WB of ERG between 1:1000 and 1:10000 - if this is your case I would go to the 1:1000 and see what happens)
Good luck
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