7 Questions 2 Answers 0 Followers
Questions related from Marian Cruz
I´ve been workin with fungi RNA, Iextracted RNA with trizol method but my RNA is contaminated with DNA, at least my tutor said that. Anybody can help me with some protocol to treat my RNA with...
06 June 2018 7,223 2 View
Hi everybody, I looking for a very good method to isolate exosomes from serum and urine. I will use these exosomes from study microRNAs. I'm never have worked with exosomes before so, I don't have...
05 May 2018 5,134 4 View
I want to quantify the expression of specific miRNAs which I extracted from paraffin tissues, but I want to quantify them and verify they expression. I don´t know if Taqman microRNA assay be the...
01 January 2018 6,107 3 View
I'm going to do a wound healing assay with a cytosine arabinoside like proliferation inhibitor, but I don't know if I have to administrate this inhibitor every 24 hours o what time is the best....
12 December 2017 965 2 View
I did a lot of Western Blot, and my band of lamin B1 always seem uneven. I charge 30ug in 20uL by well, my samples are in different conditions but lamin B1 its the control. I don´t know what can I...
02 February 2017 874 3 View
Do you know why my membrane looks like this? Do you think the membrane need wash longer or more times? I did this western for Lamin B1, and I used ERG sc-376293 from Santa Cruz as primary...
02 February 2017 8,634 4 View
I did a Western Blot yesterday but, my band doesn't exist! I think my antibody dilution is wrong... I used 1:10,000 for anti-ERG What dilution should I try this time?
10 October 2016 1,637 1 View
