We are going to use CSL as a cost-effective nitrogen source for Lactobacillus fermentation in pH 6.3. We have examined the acid treatment method to lower the pH of CSL to 3.5; after that, we centrifuged it to extract the precipitant. The problem is that when we added NaOH to adjust the pH to the optimum level of 6.3 (before or after autoclave) much more weight of precipitant formed. The situation got worse when it becomes mixed with the base culture medium. As long as our aimed product is the biomass, it is vital to remove or reduce the amount of precipitant. Have you tested any method for treating the CSL?
I like the approach but also think it would be difficult. you might study on this matter to find out the solution or find some alternative source of nitrogen supplimentation.
You can always try centrifugation or filtration after setting up the pH to 6.5 to 6.8. The supernatant you can sterilize before adding it int he final media.
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