Effect of feeding phytochemical (polyphenol) on antioxidant in blood of fish ?

Could any one share references about effect of feeding phytochemical (polyphenol) on antioxidant in blood of fish?

01 February 2018 7,142 0 View

Hydroxyproline and acid soluble collagen?

Dear every one. from results of my research, I get acid soluble collagen content was higher in sample than control, while in hydroxyproline content, Hydroxiproline did not differ between control...

01 February 2018 4,563 0 View

Antioxidant effect on collagen synthesis?

Dear! I am a student, could any one share some article or explanation about how antioxidant affect acid soluble collagen content in vivo study? Best regards Aminah

11 December 2017 6,807 0 View

Effect antioxidant on protein?

I am searching literature about The effect of dietery antioxidant on protein quality (protein degredation) Thank you.

09 October 2016 4,572 0 View

I have Olive Leaf Powder as a sample in my research, Could any one help me how to determine quantity of individual phenolic compound?

I have Olive Leaf Powder as a sample in my research, Could any one help me how to determine quantity of individual phenolic compound?

04 May 2016 9,874 2 View

Antioxidant in feed can improve collagen content in animal/fish, Can anyone explain that?

Antioxidant in feed can improve collagen content in animal/fish, Can anyone explain that?

04 May 2016 296 2 View

Hi, I am going to do my experiment, about surim?

01 January 1970 1,993 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View