Dear All
I need some literatures related to (1) effect of phenol on protein stability in surimi gel; (2) Comparison rate of protein denaturation in fish and meat and their effects on gel properties.
Dear every one. from results of my research, I get acid soluble collagen content was higher in sample than control, while in hydroxyproline content, Hydroxiproline did not differ between control...
01 February 2018 4,622 0 View
Could any one share references about effect of feeding phytochemical (polyphenol) on antioxidant in blood of fish?
01 February 2018 7,201 0 View
Dear! I am a student, could any one share some article or explanation about how antioxidant affect acid soluble collagen content in vivo study? Best regards Aminah
11 December 2017 6,853 0 View
I am searching literature about The effect of dietery antioxidant on protein quality (protein degredation) Thank you.
09 October 2016 4,629 0 View
I have Olive Leaf Powder as a sample in my research, Could any one help me how to determine quantity of individual phenolic compound?
04 May 2016 9,982 2 View
Antioxidant in feed can improve collagen content in animal/fish, Can anyone explain that?
04 May 2016 346 2 View
Hi, I need some information/literature/paper that talking about effect of polyphenol dietery on surimi quality. Coud any one help me? Best Regards
01 January 1970 2,057 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View